Quilters Furniture and Beds in Ruislip High Street has announced that the building has been sold.
The news comes 18 months after another of the borough's oldest stores, Randalls, in Uxbridge, also shut for the last time.
The firm told customers via its website and through mobile phone text messages that is having a closing down sale.
@HillingdonHERE It's really sad to hear this. I can well understand how stressful this is for the family. It's terribly sad.— Sir John Randall (@uxbridgewalrus) 20 September 2016
They have been serving the residents well over the years. Sad to hear that they are closing. They will be missed. https://t.co/w2BcQDDUi7— Mayor of Hillingdon (@HillingdonMayor) 20 September 2016
In a statement to customers, Jon Quilters said: “Since 1957, Quilters Furniture and Beds has been family owned and operated. Now, after 59 years, we have made the strategic decision to close our store and retire from the furniture business. to express our appreciation, we will have two private courtesy days, before opening our store closing sale to the general public.
“Our store is now closed to mark down prices on our entire collection of sofas, armchairs, recliners, dining rooms, bedrooms, bedding, mattresses, occasional tables, pictures, mirrors and accessories.”
Like this story? Get free email newsletters via the form below
Free email newsletter
This week the store will open to private customers for two days and it will open to the general public on Friday.
To mark the closing down sale, it will have a draw for a £500 shopping spree.
Last year, Sir John Randall, warned that Hillingdon was in danger of losing all of its traditional independent shops if residents fail to support them and spend their cash online instead.
Former Uxbridge MP Mr Randall, whose family store closed in January 2015, 124 years after his great-grandfather established it, told Hillingdon is Here at that time he had this message for all the customers who have supported Randalls throughout the years.
He said: "I would just like them to continue to support independent stores if they can. I know it's difficult not to be seduced by the internet, and I can't say that I never use internet shopping for things.
“Everybody says it's such a shame (that we're closing) but the danger is that if you are not careful we will lose them all.”